The Unique Features of UV VIS Spectroscopy
The UV VIS Spectroscopy is a special technique used widely in several areas of science, including nuclear acid purity checks, drug identification, quantification and bacterial culturing. You can also adopt it for quality control in the chemical research industry and beverage industry. The technique is analytical and advanced in its purpose. Be that as it may, it is still very easy to use. It is reliable for measuring the discrete wavelengths of visual light or ultraviolet, which informs its name. It works for visual light transmitted via or absorbed by a sample when comparing blank or reference sample its property is under the influence of sample composition too. It offers information potentially on the content of the sample and gives details of its concentration. The machine works based on the use of light. When asking what is UV VIS spectroscopy, we need to first understand the properties of light before answering the question.
Continue reading to learn more about the many features that make UV VIS spectroscopy outstanding.
How UV VIS spectroscopy work
You can find varieties of the UV VIS spectroscopy. Some of its major components are the light source, the wavelength selector, sample, detector and a computer. The light source passes through the wavelength selector, which then passes through the sample. After passing through the sample, the light goes through the detector and onward to the computer. The computer carries out the function of signal processing and will then produce the final outcome. You need to understand how it works before you can successfully provide answers to what is UV VIS spectroscopy. The light source must be stable to enable it function in emitting light across a wide range of wavelengths. You can rely on a single xenon lamp as the light source. The xenon light can even act as a source of light for both visible and UV ranges. Be that as it may, xenon lamps are costlier and not so stable. As a result, many opt for the use of halogen and tungsten lamps.
Choosing a light source
Some instruments use 2 lamps, which involves the combination of a halogen and tungsten lamps. This combination is commonly used for visible lights and deuterium lamp is the lamp of choice for UV light. Before you can scan both visible and UV wavelengths, you will require two different light sources. As a result, the source of light in the instrument needs to switch while the measurement is going on. The switch between two lamps take place while the scan is going on between 300 and 350 nanometer, which is the point where there is a similarity between the light emissions for the two sources of light. This will also smoothen the process of the transmission. The equipment does not require much maintenance also.